De Novo Mutations in CHAMP1 Cause Intellectual Disability with Severe Speech Impairment.

نویسندگان

  • Maja Hempel
  • Kirsten Cremer
  • Charlotte W Ockeloen
  • Klaske D Lichtenbelt
  • Johanna C Herkert
  • Jonas Denecke
  • Tobias B Haack
  • Alexander M Zink
  • Jessica Becker
  • Eva Wohlleber
  • Jessika Johannsen
  • Bader Alhaddad
  • Rolph Pfundt
  • Sigrid Fuchs
  • Dagmar Wieczorek
  • Tim M Strom
  • Koen L I van Gassen
  • Tjitske Kleefstra
  • Christian Kubisch
  • Hartmut Engels
  • Davor Lessel
چکیده

CHAMP1 encodes a protein with a function in kinetochore-microtubule attachment and in the regulation of chromosome segregation, both of which are known to be important for neurodevelopment. By trio whole-exome sequencing, we have identified de novo deleterious mutations in CHAMP1 in five unrelated individuals affected by intellectual disability with severe speech impairment, motor developmental delay, muscular hypotonia, and similar dysmorphic features including short philtrum and a tented upper and everted lover lip. In addition to two frameshift and one nonsense mutations, we found an identical nonsense mutation, c.1192C>T (p.Arg398*), in two affected individuals. All mutations, if resulting in a stable protein, are predicted to lead to the loss of the functionally important zinc-finger domains in the C terminus of the protein, which regulate CHAMP1 localization to chromosomes and the mitotic spindle, thereby providing a mechanistic understanding for their pathogenicity. We thus establish deleterious de novo mutations in CHAMP1 as a cause of intellectual disability.

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عنوان ژورنال:
  • American journal of human genetics

دوره 97 3  شماره 

صفحات  -

تاریخ انتشار 2015